In axons, it promotes the polarization of microtubules,

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canada goose The drug epothilone B (epoB) stabilizes microtubules, proteins in the cell that help make its shape and grow. The fascinating part is that epoB affects axons and fibroblasts differently. In axons, it promotes the polarization of microtubules, meaning they accumulate at one end of the cell and promote growth. With epoB added, the microtubules form at the tips of the cut axons and elongate them. Axons in culture, labeled in the picture above in white, exposed to epoB can even grow in the presence of inhibitory growth signals, usually secreted by fibroblasts. In fibroblasts, however, epoB halts cell growth and the polarization of microtubules. This prevents the migration of fibroblasts to the site of injury. In the picture to the right, fibroblasts are treated with epoB. After treatment, there are stable, detyrosinated microtubules (DetyrTub in green) all over the cell. Without a specific direction of microtubule growth, the cell can’t move. Without fibroblasts migrating to the injury, there is less scaring and inhibitory signaling. canada goose

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